Purification of a surface-bound hexosaminidase from spores ofBacillus cereusT
نویسندگان
چکیده
منابع مشابه
Purification, biochemical and immunological characterisation of hexosaminidase A from variant AB of infantile GM2 gangliosidosis.
Variant AB of infantile GM2 gangliosidosis is a fatal disease leading invariably to death within the first few years of life, due to the excessive storage of the glycolipids GM2 and GA2 which occurs in the nervous tissue of the patient. Unlike other variants of this hereditary disease, where a deficiency of hexosaminidase A, the ganglioside-GM2-degrading enzyme, could be demonstrated, the varia...
متن کاملPurification and properties of the hexosaminidase A-activating protein from human liver.
Human liver extracts contain an activating protein which is required for hexosaminidase A-catalysed hydrolysis of the N-acetylgalactosaminyl linkage of G(M2) ganglioside [N-acetylgalactosaminyl-(N-acetylneuraminyl) galactosylglucosylceramide]. A partially purified preparation of human liver hexosaminidase A that is substantially free of G(M2) ganglioside hydrolase activity is used to assay the ...
متن کاملEffect of Water Washing Purification on the Surface Morphology of Bacillus cereus Spores
The architecture of spore surfaces plays an important role in the pathogenesis and natural ecology of many Bacillus organisms. However, changes to the laboratory preparation method for Bacillus spores may be a source of phenotypic variability in cell surfaces that can affect our understanding of in situ morphology and make cross-study comparisons more difficult. In this work, we examined how va...
متن کاملPurification and properties of a germination-specific cortex-lytic enzyme from spores of Bacillus megaterium KM.
Two peptidoglycan-lytic enzyme activities were isolated from spores of Bacillus megaterium KM. Surface-bound lytic enzyme was extracted from dormant spores and hydrolysed a variety of peptidoglycan substrates including isolated spore cortex, but did not cause refractility changes in permeabilized spores. Germination-specific lytic enzyme activity appeared early in germination and had minimal ac...
متن کاملa study on thermodynamic models for simulation of 1,3 butadiene purification columns
attempts have been made to study the thermodynamic behavior of 1,3 butadiene purification columns with the aim of retrofitting those columns to more energy efficient separation schemes. 1,3 butadiene is purified in two columns in series through being separated from methyl acetylene and 1,2 butadiene in the first and second column respectively. comparisons have been made among different therm...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1978
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1978.tb01941.x